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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Metabolic engineering for sustainable xylitol production from diverse carbon sources in Pichia pastoris

Fig. 4

The effect of NADPH-dependent xylitol dehydrogenase via protein engineering on xylitol production. (A) Conversion of NADH-dependent xylitol dehydrogenase to NADPH-dependent xylitol dehydrogenase through site-specific mutations. (B) Yields of xylitol, xylulose, and D-arabitol by expressing of PsXyl2 mutants with KpDalD in the gsy002 strain. (C) The effect of positive PsXyl2 mutant on xylitol production in the engineered LC10 strain. (D) Deletion of native PpXyl2 increased xylitol production in PsXyl2 mutant expressing strain. All data represent the mean ± SD of biological triplicates

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Microbial Cell Factories

ISSN: 1475-2859

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