Fig. 3

Introduction of D-arabitol-dependent pathway increased xylitol production. (A) Metabolic engineering of D-arabitol-dependent pathway. (B) Xylitol production in engineered strain LC10 by expressing KpDalD. (C) Xylulose production in LC10. (D) D-arabitol production in LC10. (E) Xylitol production by copy number optimization of KpDalD and PsXyl2. Cells were cultured in 20 mL of minimal medium with 2% glucose as the sole carbon source. Samples were collected at 96 h for xylitol detection. Data represent the mean ± SD from biological triplicates