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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Metabolic engineering for sustainable xylitol production from diverse carbon sources in Pichia pastoris

Fig. 2

Construction of XU5P-dependent pathway for xylitol production. (A) Metabolic engineering of XUMP-dependent pathway. (B) Xylitol production in engineered P. pastoris strains expressing heterologous xylitol dehydrogenases and replacing xylulose kinase. (C) Deletion of native xylitol dehydrogenase encoding gene PpXyl2 in the wild-type strain abolishes xylitol production. (D) Xylulose production in engineered P. pastoris after enhancing the PPP. (E) Xylitol production by enhancing the PPP. Cells were cultured in 20 mL of minimal medium with 2% glucose as the sole carbon source. Samples were taken at 72 h, 96 h and 120 h for xylitol detection. Data represent the mean ± SD from biological triplicates

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Microbial Cell Factories

ISSN: 1475-2859

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