Table 4 Genome Integration-based secretion of functional elements in Lactobacilli
From: Metabolic engineering of Lactobacilli spp. for disease treatment
Strains | Strategy | Expression mode | Mechanisms | Diseases | Treating effect | Experimental model | Reference |
|---|---|---|---|---|---|---|---|
Secreting vaccine for treating virus infection | |||||||
L. paracaseiâ–³Alr HLJ-27 | Expressing the S1 gene of PEDV | Genome integration | Activate the mucosal, humoral, and cellular immune responses | Against PEDV | L. paracaseiâ–³Alr HLJ-27 administration could endow the piglets with resistance against PEDV LJB2019 infection by decreasing the PEDV copy number, maintaining intact villi structure, and relieving the inflammatory status | SPF BALB/c mice and large landrace piglets | [82] |
Secreting vaccine for treating pathogen infection | |||||||
L. acidophilus NCFM | Secreting the host receptor-binding domain of the heavy chain of C. botulinum serotype A and the anthrax protective antigen of B. anthracis | Genome integration | Inducing mucosal immune responses | Against C. botulinum and B. anthracis infection | [83] | ||
Secreting pharmaceutical compounds | |||||||
L. gasseri ATCC 33323 | Secreting the inactive full-length form of GLP-1(1–37) | Genome integration | Stimulating the conversion of intestinal epithelial cells into insulin-secreting cells; Increasing the insulin levels and glucose tolerant | Against diabetes | The recombinant microbe-fed diabetic rats showed high insulin levels (average 60% more total insulin (intestines and pancreases combined)) and glucose tolerance | STZ-induced diabetic rats | [85] |