Table 1 Screening results of candidate sites
From: Enhanced thermostability of nattokinase by rational design of disulfide bond
Candidate site | Distance from catalytic centre (Å) | Distance of β-carbon atoms between candidate sites (Å) | Conservation* | averageB-factor (Å) | averageRMSF (Å) |
|---|---|---|---|---|---|
T255C-L267C | 15.5 | 4.3 | 3 | 18.2 | 5.97 |
A153C-S191C | 8.0 | 4.2 | 8 | 16.92 | 5.92 |
L257C-Y263C | 15.9 | 4.2 | 1 | 18.43 | 5.69 |
R249C-A273C | 21.7 | 4.2 | 4 | 18.76 | 5.68 |
A254C-G266C | 16.9 | 4.4 | 8 | 19.72 | 5.58 |
G154Y-G166C | 7.5 | 5.5 | 9 | 20.9 | 5.51 |
N155C-F189C | 8.5 | 5.1 | 9 | 20.08 | 5.46 |
D41C-P210C | 16.9 | 11.8 | 7 | 21.24 | 5.39 |
P57C-K94C | 15.6 | 4.2 | 3 | 15.26 | 5.33 |
D41C-G80C | 18.0 | 5.0 | 7 | 16.83 | 4.81 |
D32C-G65C | 9.9 | 3.7 | 9 | 13.18 | 4.79 |
A169C-A176C | 10.7 | 3.8 | 7 | 18.32 | 4.58 |
N76C-P86C | 18.9 | 4.2 | 5 | 17.8 | 4.51 |
V95C-G110C | 15.1 | 5.2 | 6 | 15.78 | 4.42 |
I205C-M222C | 3.8 | 3.9 | 7 | 14.53 | 4.35 |
S49C-P57C | 19.0 | 4.1 | 7 | 17.02 | 4.27 |
E156C-T164C | 12.1 | 3.8 | 3 | 19.51 | 4.27 |
A179C-A223C | 5.6 | 4.2 | 7 | 14.95 | 4.22 |
G47C-P57C | 9.3 | 5.0 | 5 | 17.84 | 4.14 |
A73C-A88C | 19.0 | 4.2 | 7 | 15.1 | 4.11 |
A200C-A223C | 5.6 | 4.9 | 9 | 14.15 | 4.11 |
V26C-A232C | 17.5 | 4.2 | 7 | 14.92 | 4.02 |
L257C-G266C | 16.9 | 4.4 | 1 | 18.49 | 3.98 |
G70C-A74C | 16.3 | 3.9 | 7 | 12.55 | 3.77 |
S49C-K94C | 15.6 | 3.9 | 7 | 16.18 | 3.74 |
S33C-S62C | 12.1 | 3.5 | 7 | 17.42 | 3.55 |
H39C-L42C | 18.8 | 4.0 | 8 | 14.67 | 3.5 |
V143C-S173C | 17.9 | 4.1 | 1 | 21.08 | 3.4 |
A151C-T174C | 8.0 | 4.3 | 6 | 17.37 | 3.19 |
A254C-K265C | 18.3 | 3.8 | 8 | 20.91 | 2.96 |
G32C-D60C | 10.3 | 8.1 | 9 | 18.36 | 2.63 |
N123C-A228C | 9.4 | 3.8 | 9 | 12.02 | 2.51 |
A230C-V270C | 15.0 | 4.0 | 5 | 12.54 | 2.5 |
A114C-M119C | 19.2 | 4.2 | 4 | 17.21 | 4.25 |
W241C-Q245C | 23.8 | 4.0 | 5 | 19.22 | 2.45 |
S33C-G65C | 9.9 | 4.8 | 7 | 14.8 | 2.34 |
S37C-V44C | 19.9 | 3.9 | 1 | 16.55 | 2.31 |
N181C-V203C | 10.1 | 4.0 | 5 | 18.14 | 2.31 |
A85C-A88C | 8.5 | 3.9 | 9 | 14.25 | 2.29 |
G34C-K94C | 5.9 | 4.1 | 9 | 15.03 | 2.25 |
A15C-Q271C | 17.2 | 3.8 | 1 | 19.93 | 2.23 |
V165C-G193C | 12.5 | 5.3 | 3 | 18.41 | 2.22 |
S37C-Y58C | 19.7 | 3.9 | 1 | 18.39 | 2.21 |
F50C-N109C | 17.3 | 4.0 | 2 | 16.48 | 2.16 |
S24C-S87C | 21.4 | 4.2 | 4 | 17.12 | 1.74 |
A151C-A169C | 8.0 | 4.0 | 6 | 18.56 | 1.19 |
K12C-Q271C | 19.4 | 3.8 | 5 | 16.91 | 0.61 |
T22C-S87C | 21.4 | 3.8 | 5 | 17.23 | 0.59 |
A85C-A232C | 17.2 | 3.9 | 9 | 15.09 | 2.73 |