Development of an efficient expression system for human chaperone BiP in Pichia pastoris: production optimization and functional validation

Table 2 The purification process of rhBiP from P. pastoris culture medium

Purification step	Sample Volume, ml	rhBiP concentration, mg/ml	rhBiP quantity, mg	Purity according to SDS-PAGE, %	Recovery, %
Supernatant	1050	0.0666	70	28	100
Phenyl chromatography	120	0.49	58.8	64	84
Buffer exchange with sephadex G25	220	0.267	58.8	64	84
First sepharose Q	25	2.068	51.7	81	73.9
Second sepharose Q	30	1.51	45.3	90	64.7
Buffer exchange with sephadex G25	45	1	45	90	64

ISSN: 1475-2859