Table 1 Optimization of rhBiP secretion in the mineral culture medium in shake flasks
Growth media | rhBiP yield (titer), mg/L after 42 h induction | WCW, g/L after 42 h induction | Induction medium components |
|---|---|---|---|
YEPM | 11.8 ± 1.6 | 65.7 ± 3.7 | 2% soy peptone, 1% yeast extract, 0.5% methanol |
BSM | 2.2 ± 1.0 | 53.2 ± 6.9 | ¼ BSM salts, 100 mM potassium phosphate, 0.5% methanol |
BSM + 2 mM DTT | 7.7 ± 2.2 | 56.0 ± 1,4 | ¼ BSM salts, 100 mM potassium phosphate, 2 mM DTT, 0.5% methanol |
BSM + 2 mM TCEP | 8.8 ± 1.3 | 56.5 ± 3.5 | ¼ BSM salts, 100 mM potassium phosphate, 2 mM TCEP, 0.5% methanol |
BSM + 0.75% Glucose | 7.9 ± 3.4 | 74.5 ± 3.3 | ¼ BSM salts, 100 mM potassium phosphate, 0.75% glucose, 0.5% methanol |
BSM + 2 mM DTT + 0,75% Glucose | 17.2 ± 0.7 | 72.4 ± 2.3 | ¼ BSM salts, 100 mM potassium phosphate, 2 mM DTT, 0.75% glucose, 0.5% methanol |