Fig. 3

Secretion of rhBiP in high-cell-density fermentation of P. pastoris in a bioreactor. (A) SDS-PAGE analysis of culture supernatant during fermentation. 35 µl of P. pastoris clone ppBiP10 culture media, taken at different time points, was loaded onto each lane. (B) Time-course profile of rhBiP secretion in high-density P. pastoris culture during fermentation. Circles represent wet cell weight (WCW), and triangles show the concentration of the secreted rhBiP in the culture medium. 0–24 h indicates glycerol batch phase, 24–25 h - glycerol fed-batch phase, 25–28 h - transition phase, and 28–100 h - methanol/glucose fed-batch phase. Only the amount of intact secreted rhBiP protein form was calculated