Fig. 4

Genomic characterization of strains with increased laccase activity. (A) Number of ORFs that are either significantly enriched or depleted in the 20 strains with higher laccase activity. We first identified genes that are either present or absent in any of the 20 hit strains in comparison to the reference strain, BY4741, and then used Fisher’s exact test to determine whether these genes are statistically enriched or depleted in comparison to the whole strain library. The compensated group consists of 10 depleted genes for which a similar ORF is also enriched. (B) Cell map of the localization of known genes or of their close homolog. Enriched ORFs shown in blue, depleted in green and compensated lighter colours. (C, D and E) Dot plots of the compensated and depleted genes groups, indicating Biological Process or Cellular Component GO terms enriched in each group. The scale of the false discovery rate (FDR) q-values are shown in (E, F) Select gene targets from genomic analysis were knocked out and tested for their effect on ttLcc1 activity after 4-days growth. BY4741 was used as the control and reference for statistical tests. Significantly higher or lower laccase activities indicated in green or in grey, respectively (p-values: * < 0.05, ** < 0.01 *** < 0.001, **** < 0.0001)