Skip to main content
Fig. 3 | Microbial Cell Factories

Fig. 3

From: Mining yeast diversity unveils novel targets for improved heterologous laccase production in Saccharomyces cerevisiae

Fig. 3

Probing the contribution of transcription and glycosylation toward increased ttlcc1 activity. (A) Relative ttLCC1 mRNA levels of the indicated strains. After 4-days growth, an equivalent of 25 OD600 of cells were processed for mRNA purification and RT-qPCR (n = 3). Levels of ttLCC1 mRNA were normalized to UBC6. (B) Laccase activity relative to the averaged ttLCC1 mRNA levels for each strain in shown. Laccase activity was measured in parallel to mRNA quantitation. (C) ttLcc1 activity after no treatment, mock and PNGase F treatment (p-values: * < 0.05, ** < 0.01 *** < 0.001, **** < 0.0001)

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us