Fig. 5
Supplementation with organic nitrogen sources alleviated the growth defect of mnn11Δ mutant. (A-E) Growth curves of wild-type and mnn11Δ cells with different nitrogen sources. Cells were inoculated into 50 mL medium at an initial OD600 of 0.01 and grown at 30 °C for 72 h. Growth curves were obtained by monitoring OD600 in YD and SMS medium (A), SMS medium supplemented with 0.1% valine or tyrosine (B), 0.1% lysine or arginine (C), 0.1% methionine or glutamate (D), and different concentrations of yeast extract (YE) (E). Growth curves of wild-type cells in (B, C, D) were from the same sample. Values were calculated as mean ± SD (n = 3) from biological replicates. The maximum specific growth rate (µmax) for each curve is shown in the inset. (F) Spot assay of wild-type and mnn11Δ cells. Overnight cultures were diluted 5-fold and spotted onto YD, SMS, and SMS plates supplemented with 0.5% YE. To test cell wall integrity, the medium was supplemented with hygromycin B. Plates were incubated at 30 °C for 24 h