Table 5 Design of different trials of the RSM for independent variables and responses by mutant P. aeruginosa
Run | pH | Temp | Tween 80 | Peptone | Olive oil conc | Incub. time | Response (U/ml) |
|---|---|---|---|---|---|---|---|
1 | 9 | 55 | 0.5 | 0.5 | 3 | 72 | 496.25 |
2 | 7 | 37.5 | 1.5 | 1.5 | 3 | 48 | 411.26 |
3 | 7 | 37.5 | 1.5 | 1.5 | 2 | 48 | 410.08 |
4 | 9 | 55 | 0.5 | 2.5 | 3 | 72 | 495.37 |
5 | 9 | 55 | 2.5 | 2.5 | 3 | 24 | 496.41 |
6 | 9 | 20 | 0.5 | 0.5 | 3 | 24 | 497.43 |
7 | 5 | 20 | 2.5 | 2.5 | 3 | 72 | 363.22 |
8 | 5 | 55 | 0.5 | 0.5 | 1 | 24 | 361.92 |
9 | 5 | 55 | 2.5 | 0.5 | 3 | 72 | 366.45 |
10 | 5 | 55 | 2.5 | 2.5 | 1 | 24 | 364.56 |
11 | 7 | 37.5 | 1.5 | 1.5 | 2 | 48 | 413.49 |
12 | 5 | 20 | 2.5 | 2.5 | 1 | 24 | 366.46 |
13 | 9 | 55 | 0.5 | 2.5 | 3 | 24 | 491.23 |
14 | 9 | 20 | 2.5 | 2.5 | 1 | 72 | 497.44 |
15 | 7 | 37.5 | 1.5 | 1.5 | 2 | 48 | 412.39 |
16 | 5 | 20 | 2.5 | 0.5 | 1 | 24 | 361.21 |
17 | 9 | 55 | 0.5 | 0.5 | 1 | 72 | 493.39 |
18 | 9 | 55 | 2.5 | 0.5 | 1 | 24 | 490.21 |
19 | 5 | 55 | 2.5 | 0.5 | 1 | 72 | 365.38 |
20 | 7 | 37.5 | 1.5 | 1.5 | 2 | 48 | 414.47 |
21 | 9 | 37.5 | 1.5 | 1.5 | 2 | 48 | 494.01 |
22 | 9 | 55 | 2.5 | 0.5 | 3 | 24 | 496.49 |
23 | 5 | 20 | 0.5 | 0.5 | 3 | 24 | 367.48 |
24 | 9 | 20 | 0.5 | 0.5 | 3 | 72 | 496.84 |
25 | 7 | 37.5 | 1.5 | 1.5 | 2 | 48 | 416.95 |
26 | 5 | 55 | 2.5 | 2.5 | 3 | 72 | 363.49 |
27 | 9 | 20 | 2.5 | 0.5 | 3 | 24 | 492.56 |
28 | 5 | 20 | 2.5 | 2.5 | 3 | 24 | 368.49 |
29 | 9 | 55 | 0.5 | 0.5 | 3 | 24 | 496.21 |
30 | 5 | 20 | 0.5 | 2.5 | 1 | 24 | 363.33 |
31 | 7 | 37.5 | 1.5 | 1.5 | 2 | 48 | 414.46 |
32 | 9 | 20 | 2.5 | 2.5 | 3 | 72 | 491.26 |
33 | 5 | 37.5 | 1.5 | 1.5 | 2 | 48 | 365.53 |
34 | 9 | 55 | 2.5 | 0.5 | 3 | 72 | 490.47 |
35 | 7 | 37.5 | 1.5 | 1.5 | 2 | 48 | 413.22 |
36 | 9 | 55 | 0.5 | 2.5 | 1 | 24 | 493.56 |
37 | 9 | 20 | 2.5 | 0.5 | 1 | 24 | 495.31 |
38 | 5 | 20 | 2.5 | 2.5 | 1 | 72 | 366.49 |
39 | 5 | 55 | 2.5 | 2.5 | 1 | 72 | 363.26 |
40 | 7 | 37.5 | 0.5 | 1.5 | 2 | 48 | 415.53 |
41 | 7 | 37.5 | 1.5 | 1.5 | 2 | 48 | 410.31 |
42 | 5 | 20 | 0.5 | 0.5 | 1 | 72 | 367.74 |
43 | 9 | 20 | 2.5 | 2.5 | 3 | 24 | 493.29 |
44 | 9 | 55 | 2.5 | 0.5 | 1 | 72 | 495.42 |
45 | 7 | 20 | 1.5 | 1.5 | 2 | 48 | 409.37 |
46 | 5 | 55 | 2.5 | 0.5 | 1 | 24 | 368.46 |
47 | 9 | 55 | 2.5 | 2.5 | 1 | 24 | 490.77 |
48 | 5 | 55 | 2.5 | 2.5 | 3 | 24 | 362.26 |
49 | 9 | 55 | 2.5 | 2.5 | 3 | 72 | 491.32 |
50 | 7 | 37.5 | 2.5 | 1.5 | 2 | 48 | 414.54 |
51 | 5 | 20 | 0.5 | 2.5 | 1 | 72 | 365.88 |
52 | 7 | 55 | 1.5 | 1.5 | 2 | 48 | 412.11 |
53 | 9 | 20 | 0.5 | 2.5 | 3 | 72 | 494.45 |
54 | 9 | 20 | 0.5 | 2.5 | 1 | 24 | 491.59 |
55 | 9 | 55 | 0.5 | 2.5 | 1 | 72 | 490.29 |
56 | 7 | 37.5 | 1.5 | 1.5 | 2 | 72 | 415.83 |
57 | 9 | 55 | 2.5 | 2.5 | 1 | 72 | 498.25 |
58 | 7 | 37.5 | 1.5 | 0.5 | 2 | 48 | 414.01 |
59 | 9 | 20 | 0.5 | 2.5 | 3 | 24 | 492.36 |
60 | 5 | 55 | 2.5 | 0.5 | 3 | 24 | 366.11 |
61 | 7 | 37.5 | 1.5 | 1.5 | 2 | 48 | 413.08 |
62 | 5 | 20 | 2.5 | 0.5 | 1 | 72 | 362.33 |
63 | 5 | 20 | 0.5 | 2.5 | 3 | 24 | 364.39 |
64 | 7 | 37.5 | 1.5 | 1.5 | 2 | 24 | 419.41 |
65 | 9 | 20 | 0.5 | 0.5 | 1 | 24 | 495.39 |
66 | 5 | 55 | 0.5 | 0.5 | 3 | 72 | 369.87 |
67 | 5 | 20 | 2.5 | 0.5 | 3 | 72 | 360.55 |
68 | 5 | 20 | 0.5 | 0.5 | 1 | 24 | 361.22 |
69 | 5 | 20 | 0.5 | 0.5 | 3 | 72 | 365.45 |
70 | 5 | 55 | 0.5 | 2.5 | 1 | 72 | 366.93 |
71 | 9 | 20 | 0.5 | 2.5 | 1 | 72 | 493.89 |
72 | 5 | 20 | 0.5 | 2.5 | 3 | 72 | 367.71 |
73 | 5 | 55 | 0.5 | 0.5 | 1 | 72 | 365.34 |
74 | 9 | 20 | 2.5 | 2.5 | 1 | 24 | 499.33 |
75 | 7 | 37.5 | 1.5 | 1.5 | 1 | 48 | 414.39 |
76 | 7 | 37.5 | 1.5 | 1.5 | 2 | 48 | 410.99 |
77 | 9 | 20 | 2.5 | 0.5 | 1 | 72 | 497.72 |
78 | 5 | 55 | 0.5 | 2.5 | 3 | 72 | 362.74 |
79 | 7 | 37.5 | 1.5 | 2.5 | 2 | 48 | 411.13 |
80 | 5 | 55 | 0.5 | 2.5 | 3 | 24 | 364.44 |
81 | 9 | 20 | 0.5 | 0.5 | 1 | 72 | 496.37 |
82 | 9 | 55 | 0.5 | 0.5 | 1 | 24 | 495.52 |
83 | 5 | 55 | 0.5 | 0.5 | 3 | 24 | 367.72 |
84 | 9 | 20 | 2.5 | 0.5 | 3 | 72 | 493.24 |
85 | 5 | 20 | 2.5 | 0.5 | 3 | 24 | 360.84 |
86 | 5 | 55 | 0.5 | 2.5 | 1 | 24 | 365.98 |