Fig. 2

Overview of the fundamental procedure used to generate E. coli lysate for phage directed CFES. The conditions shown are those used for E. coli given that it is the only organism used so far in phage production. (1) Pre-lysis step: which entails streaking of the organism from stock, pre-culturing in yeast extract tryptone/phosphates glucose media (2xYTP-G), culturing, harvesting and finally washing with S30A buffer. (2) Cell lysis step: this step involves lysing the bacterium to release the cellular machinery. The lysozyme assisted sonication method is highlighted. (3) Post-lysis step: final processing of the extract involves centrifugation, a runoff reaction, then dialysis with the S30B buffer, and storage for long term usage. Figure is generated using BioRender