Fig. 2

Comparison of mupirocin ribosome profiling data by counting from either 3’ or 5’ end of RPF reads. (A) Schematic diagram for calculating the position of the first nucleotide in the ribosomal A-site codon from an RPF fragment from mupirocin treated cells when counting from either the 3’ or 5’ end of RPFs. In this example the ribosomal A-site contains the isoleucine codon ATC. (B) A-site distance distribution analysis. Average RPF reads of calculated A-sites for every nucleotide upstream and downstream of isoleucine codons. 0 indicates the first nucleotide of the calculated A-site of isoleucine codons. The A-site was determined by counting from either the 3’ end (offset 12 nt) or 5’ end (offset 14 nt). The advantage of this method is that it also indicates whether the offset used (12 and 14 nt) gives the best score