Fig. 2

Microscopic images of wild type and NosPHB1.0 strain (a) Wildtype Nostoc sp. 7120 (b) Recombinant Nostoc strain NosPHB1.0. PHB granules are visualized with BODIPY staining (GFP channel), autofluorescence (CY3 channel), overlay of phase contrast, GFP and CY3 channel, scale bar = 10 μm; characterization of NosPHB1.0 (c) Growth curve of wild-type strain Nostoc sp. PCC7120 (black) and NosPHB1.0 (gray), recorded by measuring the OD750. Longer ticks on the x-axis indicate samples taken for PHB quantification. Each point represents one biological replicate (d) PHB content of NosPHB1.0 after 3, 5 and 6 days of constant growth conditions. Each data set represents duplicates of the same biological sample