Physiology-informed use of Cupriavidus necator in biomanufacturing: a review of advances and challenges

Table 1 Available tools for engineering C. necator

Tool	Use	Purpose	References
Bioconjugation	Experimental	Transformation	[81]
Chemical transformation	Experimental	Transformation	[82]
Electroporation	Experimental	Transformation	[81, 82, 84]
pCAT vector series	Experimental	Plasmid	[82]
pMTL70000 vector series	Experimental	Plasmid	[85]
Tn5 transposon	Experimental	Genome engineering (random integration)	[56]
RalstTron	Experimental	Genome engineering (target gene knockout)	[56]
Homologous recombination + suicide plasmid	Experimental	Genome engineering (targeted gene knockout)	[56]
CRISPR-Cas9	Experimental	Genome engineering	[84]
CRAGE	Experimental	Genome engineering	[20, 91]
CIFR	Experimental	Genome engineering	[92]
Constitutive promoters	Experimental	Regulation	[93, 94]
RBSs	Experimental	Regulation	[64]
CRISPRi	Experimental	Regulation	[64]
ALE	Experimental	Controlled mutation	[42, 64]
Sequenced genome	Computational	Identifying genetic features	[30]
GSM	Computational	FBA, RBA	[40, 100, 103]

ISSN: 1475-2859