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Table 3 Taguchi design of the selected factors for lipid production by C. parapsilosis Y19 strain

From: Response surface methodology and repeated-batch fermentation strategies for enhancing lipid production from marine oleaginous Candida parapsilosis Y19 using orange peel waste

Run no.

Orange Peel (g/l)

Peptone (g/l)

Yeast extract (g/l)

Inoculum size (%, v/v)

Initial pH

incubation period (days)

Biomass Conc.

(g/l)

Total lipids

(g/l)

Lipid content %

1

50

1

1

2

4

2

3.60

0.50

13.89

2

50

3

2

4

5

4

4.00

1.44

36.00

3

50

5

3

6

6

6

4.80

1.00

20.83

4

50

7

4

8

7

8

8.80

0.98

11.14

5

50

9

5

10

8

10

12.00

1.98

16.50

6

75

1

2

6

7

10

5.20

1.60

30.77

7

75

3

3

8

8

2

7.20

1.00

13.89

8

75

5

4

10

4

4

4.80

1.02

21.25

9

75

7

5

2

5

6

8.00

2.46

30.75

10

75

9

1

4

6

8

7.20

2.02

28.06

11

100

1

3

10

5

8

9.60

0.98

10.21

12

100

3

4

2

6

10

10.00

1.44

14.40

13

100

5

5

4

7

2

6.80

0.98

14.41

14

100

7

1

6

8

4

5.20

1.00

19.23

15

100

9

2

8

4

6

4.80

1.22

25.42

16

125

1

4

4

8

6

6.00

1.46

24.33

17

125

3

5

6

4

8

14.00

1.50

10.71

18

125

5

1

8

5

10

10.40

1.48

14.23

19

125

7

2

10

6

2

6.40

1.32

20.63

20

125

9

3

2

7

4

8.40

2.00

23.81

21

150

1

5

8

6

4

8.00

0.96

12.00

22

150

3

1

10

7

6

8.40

1.30

15.48

23

150

5

2

2

8

8

12.00

2.28

19.00

24

150

7

3

4

4

10

5.60

1.50

26.79

25

150

9

4

6

5

2

4.00

1.74

43.50