Fig. 2

The changes of the HA MW after knockout of the hasE gene. The gel electrophoresis of HA (A) and HA MW (B) of the S12 and SE0 (S12 ΔhasE) after 24 h of culture using 20 g/L sucrose as the sole carbon source. The gel electrophoresis of HA (C), HA MW and hasE transcription levels (D) of the WT, SE1 and SE2 after 24 h of culture using 20 g/L sucrose as carbon source. The gel electrophoresis of HA (E), HA MW and hasE transcription levels (F) of the WT and SE3 after 24 h of culture feeding 20 g/L fructose as carbon source, adding 3 g/L and 5 g/L of sucrose as inducers, respectively