Table 1 Bacterial strains and plasmids used in the study
From: H2-driven xylitol production in Cupriavidus necator H16
Relevant characteristics | Source or reference | |
|---|---|---|
Strain | ||
C. necator H16 △phaCAB | △phaC1 △phaA1 △phaB1 | [27] |
C. necator H16 △phaC1 | △phaC1 | [28] |
C. necator H16 △A0006 | △phaC1 △A0006 | This study |
C. necator H16 △phaCAB_gfp | △phaCAB derivative, pPj5:GFP | This study |
C. necator H16 △phaC_gfp | △A0006 derivative, pPj5:GFP | This study |
C. necator H16 △phaCAB_xr | △phaCAB derivative, pPj5:XRsti | This study |
C. necator H16 △phaC_xr | △A0006 derivative, pPj5:XRsti | This study |
Plasmid | ||
pLO3 | Suicide vector, sacB | [29] |
pLO3-A0006 | pLO3 derivative with 1100 bp upstream and downstream regions of A0006 | This study |
pPj5:GFP | pSEVA521 containing Pj5 promoter and GFP | [30] |
pPj5:XRsti | pPj5:GFP derivative, GFP replaced with d-xylose reductase from Scheffersomyces stipitis (XRsti) | This study |