Table 1 Purification of pectinase from Aspergillus nidulans
Purification step | Total protein (mg) | Total activity (U) | Specific activity (Umg−1 protein) | Yield (%) | Fold of purification |
|---|---|---|---|---|---|
Crude extract | 122 | 395 | 3.2 | 100 | 1.0 |
85% (NH4)2 SO4 | 54.0 | 280 | 5.0 | 70.9 | 1.6 |
DEAE-cellulose | 1.3 | 160 | 123.1 | 40.5 | 38.5 |
Sephadex G-200 | 0.3 | 120 | 400.0 | 30.4 | 125 |