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Table 1 Genes in the vicinity of vanA and vanB in NGC7

From: Engineering a vanillate-producing strain of Pseudomonas sp. NGC7 corresponding to aromatic compounds derived from the continuous catalytic alkaline oxidation of sulfite lignin

Locus tag

Similar proteina

Organism

Accession No.

Identityb

vanA1B1, vanA2B2

    

PSN_2690

Gallate transporter

P. putida

E8ZB61.1

29

PSN_2691 (vanB1)

Carnitine monooxygenase reductase subunit

Acinetobacter baumannii ATCC 19606

D0C9N8.1

28

PSN_2692 (vanA1)

Vanillate O-demethylase oxygenase subunit

Pseudomonas sp. HR199

O05616.1

42

PSN_2693

Gallate dioxygenase

P. putida KT2440

Q88JX5.1

17

PSN_2694

Protocatechuate 4,5-dioxygenase beta chain

S. lignivorans SYK-6

P22636.1

41

PSN_2695 (vanA2)

Toluene-4-sulfonate monooxygenase system iron-sulfur subunit TsaM1

Comamonas testosteroni

P94679.1

31

PSN_2696 (vanB2)

Putative toluene-4-sulfonate monooxygenase system reductase subunit TsaB2

C. testosteroni

Q9AHG2.1

38

PSN_2697

Uncharacterized HTH-type transcriptional regulator YdhC

Bacillus subtilis 168

O05494.1

23

vanA3B3

    

PSN_3258

HTH-type transcriptional regulator PcaQ

Agrobacterium fabrum C58

P0A4T6.1

28

PSN_3259 (vanA3)

Putative toluene-4-sulfonate monooxygenase system iron-sulfur subunit TsaM2

C. testosteroni

Q9AHG3.1

35

PSN_3260 (vanB3)

Vanillate O-demethylase oxidoreductase

Pseudomonas sp. HR199

O05617.1

56

PSN_3261

Not found

-

  

vanA4B4

    

PSN_3753

Flagellar basal-body rod protein FlgG

Buchnera aphidicola Sg

Q8K9K4.1

14

PSN_3754 (vanA4)

Vanillate O-demethylase oxygenase subunit

Pseudomonas sp. HR199

O05616.1

78

PSN_3755 (vanB4)

Vanillate O-demethylase oxidoreductase

P. putida

O54037.1

93

PSN_3756

Uncharacterized HTH-type transcriptional regulator YdhC

Bacillus subtilis 168

O05494.1

27

PSN_3757

Gallate transporter

P. putida

E8ZB61.1

35

vanB5

    

PSN_3718

FMN reductase (NADH) RutF

Ancylobacter novellus DSM 506

D7A989.1

44

PSN_3719 (vanB5)

Vanillate O-demethylase oxidoreductase

Pseudomonas sp. HR199

O05617.1

50

PSN_3720

3-oxoacyl-[acyl-carrier-protein] reductase FabG

Staphylococcus epidermidis RP62A

Q5HPW0.1

34

vanB6

    

PSN_5572

Porin-like protein NicP

P. putida KT2440

Q88FY7.1

34

PSN_5573 (vanB6)

Vanillate O-demethylase oxidoreductase

P. putida

O54037.1

50

PSN_5574

Not found

-

  
  1. a Best-hit gene products from similarity search using BLAST program against UniProtKB/Swiss-Prot database are shown
  2. b Each deduced amino acid sequence was compared using the EMBOSS Needle program
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Microbial Cell Factories

ISSN: 1475-2859

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