A novel engineered strain of Methylorubrum extorquens for methylotrophic production of glycolic acid

Table 3 Summary of (by-)product titers and volumetric productivities of the initial M. extorquens TK 0001 GA producer strain Mea-GA1 and second generation strains Mea-GA2 and Mea-GA3

Strain	GX Feed	GA_max [g_GA L⁻¹]	LA [g_LA L⁻¹]	Q_GA [mg_GA L⁻¹ h⁻¹]	Q_LA [mg_LA L⁻¹ h⁻¹]
Mea-GA1	−	0.24	0.18	20.17	15.03
Mea-GA1	+	1.04	0.32	44.34	13.64
Mea-GA2	−	0.26	0.30	11.36	13.01
Mea-GA2	+	0.53	0.26	21.34	10.41
Mea-GA3	−	0.15	0.18	13.04	15.04
Mea-GA3	+	0.64	0.46	23.13	16.73

The maximal glycolic acid (GA_max) and the corresponding lactic acid (LA) titer are given as mean of independent biological duplicates. Volumetric productivities Q_GA and Q_LA were determined over the time period from induction until reaching GA_max. Strains were cultivated without (−) and with supplementation of 1.5 g L⁻¹ glyoxylate ( +) to the medium 1 h after induction time point (GX feed)

ISSN: 1475-2859