Fig. 1
Metabolic engineering strategies for acetate production from corn stover hydrolysate by E. coli BL21 (DE3). PTS, phosphotransferase system; GalP, galactose permease; XylE, xylose transporter; XylFGH, xylose ABC transporter; ptsG, glucose-specific transporter EIICBGlc coding gene; glK, glucose kinase coding gene; xylA, xylose isomerase coding gene; xylB, xylulose kinase coding gene; zwf, glucose-6-phosphate dehydrogenase coding gene; pgl, 6-phosphogluconolactonase coding gene; gnd, 6-phosphogluconate dehydrogenase coding gene; rpe, ribulose-5-phosphate 3-epimerase coding gene; pgi, glucose-6-phosphate isomerase coding gene; edd, 6-phosphogluconate dehydratase coding gene; fxpk, phosphoketolase coding gene; fbp, fructose-1,6-bisphosphatase coding gene; tal, transaldolase coding gene; tkt, transketolase coding gene; rpi, ribulose-5-phosphate epimerase coding gene; pfkA/pfkB, 6-phosphofructokinase coding gene; fba, fructose-1,6-bisphosphate aldolase coding gene; tpiA, triosephosphate isomerase coding gene; ldhA, lactate dehydrogenase coding gene; adhE, acetaldehyde/alcohol dehydrogenase coding gene; frdA, fumarate reductase subunit A coding gene; pta, phosphate acetyltransferase coding gene; ackA, acetate kinase coding gene; poxB, pyruvate oxidase coding gene. Abbreviations for metabolites: G6P, glucose-6-phosphate; 6PGL, gluconolactone-6-phosphate; 6PG, 6-phosphogluconate; Ru5P, ribulose-5-phosphate; X5P, xylulose-5-phosphate; 2KDPG, 2-keto-3-deoxy-6-phosphogluconate; F6P, fructose-6-phosphate; FBP, fructose-1,6-bisphosphate; AcP, acetyl-phosphate; E4P, erythrose-4-phosphate; S7P, sedoheptulose-7-phosphate; G3P, glyceraldehyde-3-phosphate; R5P, ribose-5-phosphate; DHAP, dihydroxyacetone phosphate