Fig. 4

Negative regulation of AbrB on nitrate respiration. A Relative transcription levels of nitrate respiration genes at 8–9 h of cultivation. B Relative transcription levels in M9 medium with nitrate addition at 8–9 h of cultivation. C EMSA of AbrB with PnarK (-253 to + 219). D EMSA of AbrB with PnarG (-200 to + 103). E EMSA of AbrB with Pfnr (-259 to + 47). F Transcriptional regulation map of nitrate respiration mediated by AbrB