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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Understanding energy fluctuation during the transition state: The role of AbrB in Bacillus licheniformis

Fig. 2

Positive regulation of AbrB on substrate level phosphorylation. A Relative transcription levels of substrate level phosphorylation genes at 8–9 h of cultivation. B EMSA of AbrB with Ppgk (-252 to + 118). C EMSA of AbrB with Ppyk (-282 to + 88). D EMSA of AbrB with PccpN (-322 to + 48). Lane 1, no protein; lane 2, 20 µM AbrB; lane 3, 40 µM AbrB; lane 4, 20 µM AbrB and 1 µg non-specific competitive DNA poly(dI: dC); lane 5, 20 µM AbrB and 200 ng DNA probe without biotin label. E Regulation map of substrate level phosphorylation by AbrB

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Microbial Cell Factories

ISSN: 1475-2859

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