Table 2 Amino acid substitutions observed in the evolved strains

Missense genetic mutations

 534,297

cg0596

rp1D

50 S ribosomal protein L4

209 A→G

K70R

 825,222

cg0892

Hypothetical protein

377 C→A

P126H

 870,566

cg0938

cspB

Cold shock protein

158 C→G

A53G

 880,237

cg0949

gltA

Citrate synthase

929G→A

R310H

 1,383,111

cg1487

leuC

3-Isopropylmalate dehydratase large subunit

1205 C→T

A402V

 1,960,344

cg2066

Uncharacterized low-complexity protein

469 A→G

S157G

 1,995,801

cg2106

Hypothetical protein

331G→A

E111K

 2,396,549

cg2516

hrcA

Transcriptional regulator of heat shock genes

67T→C

S23P

 2,396,260

cg2516

hrcA

Transcriptional regulator of heat shock genes

356T→C

L119P

 2,495,766

cg2614

TetR-family transcription regulator

530T→A

L177H

 2,606,120

cg2737

fasR

Bacterial regulatory protein, tetR family

304 C→T

L102F

 2,936,638

cg3078

Hypothetical protein

265G→A

E89K

 2,959,514

cg3100

dnaK

Heat shock protein hsp70

190 C→T

R64C

Same-sense genetic mutations

 1,960,333

cg2066

Uncharacterized low-complexity protein

480G→A

S160S

 1,960,441

cg2066

Uncharacterized low-complexity protein

372 A→G

K124K

 275,631

cg0310

katA

Catalase

1308 C→T

H436H