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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Thermo-adaptive evolution of Corynebacterium glutamicum reveals the regulatory functions of fasR and hrcA in heat tolerance

Fig. 3

Response mechanism to heat shock exhibited by the hrcA-L119P. (A) Schematic drawing to illustrate hrcA regulatory mechanism in C. glutamicum. (B) Relative mRNA levels of the heat shock protein (HSP) genes encoding molecular chaperones in wild-type ATCC 13032 and hrcA-L119P mutant at 32 °C and 42 °C. Total RNAs were prepared from cells grown to the early exponential phase in LBHIS medium. Aliquots of RNAs were reverse transcribed and subjected to qPCR. The transcript levels in wild-type ATCC 13032 were set to 1.0. Data represent mean values from three independent cultures

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Microbial Cell Factories

ISSN: 1475-2859

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