From: Engineering of bacteria towards programmed autolysis: why, how, and when?
Bacterium | Lytic enzyme/protein | Product | Ref. |
---|---|---|---|
Pseudomonas putida | Ejh (holin) and Ejl (endolysin) from pneumococcal phage EJ-1 | Polyhydroxyalkanoates (PHA) | [27] |
Escherichia coli | Lytic cassette SRRz from E. coli phage lambda with an amber mutation of S | PHA | [28] |
Escherichia coli | Lytic cassette SRRz from E. coli phage lambda | β-Glucosidase, β-galactosidase, amadoriase, GFP, lipase | |
Halomonas campaniensis | Lytic cassette SRRz from E. coli phage lambda | GFP | [20] |
Synechocystis sp. | Lytic cassette GP13 15 19 from Salmonella phage P22 and lytic cassette SRRz from E. coli phage lambda | Membrane lipids | [24] |
Bacillus megaterium | Holin-endolysin from Bacillus amyloliquefaciens phage | PHA | [25] |
Escherichia coli | Holin-endolysin from E. coli phage T4 | Red fluorescent protein | [26] |
Escherichia coli | Holin of T4 phage, T7 lysozyme | β-Glucuronidase, β-galactosidase | |
Escherichia coli | D-Amino acid oxidase, T7 lysozyme | GFP | [34] |
Escherichia coli | Endolysin R from E. coli phage lambda | GFP | |
Escherichia coli | VanX, a D-Ala-D-Ala dipeptidase | GFP, luciferase | [38] |
Escherichia coli | T7 lysozyme from E. coli phage T7 | PHA | [31] |
Escherichia coli | T4 lysozyme with N-terminal fusion of two copies of an amphipathic cell-penetrating peptide | Lysostaphin, GFP, trehalose synthase | |
Escherichia coli | T7 lysozyme with N-terminal fusion of FhuD signal peptide for secretion and C-terminal fusion of SsrA tag for recognition by ClpX protease | GFP, human growth hormone, human necrosis factor α, α amylase | [41] |
Escherichia coli, Pseudomonas putida | C-Type lysozyme with N-terminal fusion of a signal peptide for secretion | PHA | [40] |
Escherichia coli | Toxicity domain of colicin M with N-terminal fusion of PelB signal peptide and SsrA degradation tag | Poly(lactate-co-3-hydroxybutyrate), acetate | [8] |
Escherichia coli | Chimeric enzyme ClyN targeting streptococci | Enzyme mutants | [47] |
Salmonella enterica serovar Typhimurium | Protein E from E. coli phage X174 | Haemolysin E, GFP, cyan fluorescent protein | |
Escherichia coli | Protein E from E. coli phage X174 | PHA, GFP, β-galactosidase | |
Synechocystis sp. | Lipase from Fervidobacterium nodosum Rt17-B1 | Membrane lipids | [58] |
Synechocystis sp. | Lipase from Staphylococcus hyicus, phospholipase from Fusarium oxysporum, or lipase from guinea pig | Membrane lipids | [59] |