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Table 1 List of lytic proteins/enzymes used for cell autolysis and release of various products

From: Engineering of bacteria towards programmed autolysis: why, how, and when?

Bacterium

Lytic enzyme/protein

Product

Ref.

Pseudomonas putida

Ejh (holin) and Ejl (endolysin) from pneumococcal phage EJ-1

Polyhydroxyalkanoates (PHA)

[27]

Escherichia coli

Lytic cassette SRRz from E. coli phage lambda with an amber mutation of S

PHA

[28]

Escherichia coli

Lytic cassette SRRz from E. coli phage lambda

β-Glucosidase, β-galactosidase, amadoriase, GFP, lipase

[18,19,20, 22, 23]

Halomonas campaniensis

Lytic cassette SRRz from E. coli phage lambda

GFP

[20]

Synechocystis sp.

Lytic cassette GP13 15 19 from Salmonella phage P22 and lytic cassette SRRz from E. coli phage lambda

Membrane lipids

[24]

Bacillus megaterium

Holin-endolysin from Bacillus amyloliquefaciens phage

PHA

[25]

Escherichia coli

Holin-endolysin from E. coli phage T4

Red fluorescent protein

[26]

Escherichia coli

Holin of T4 phage, T7 lysozyme

β-Glucuronidase, β-galactosidase

[6, 39]

Escherichia coli

D-Amino acid oxidase, T7 lysozyme

GFP

[34]

Escherichia coli

Endolysin R from E. coli phage lambda

GFP

[29, 30]

Escherichia coli

VanX, a D-Ala-D-Ala dipeptidase

GFP, luciferase

[38]

Escherichia coli

T7 lysozyme from E. coli phage T7

PHA

[31]

Escherichia coli

T4 lysozyme with N-terminal fusion of two copies of an amphipathic cell-penetrating peptide

Lysostaphin, GFP, trehalose synthase

[2, 46]

Escherichia coli

T7 lysozyme with N-terminal fusion of FhuD signal peptide for secretion and C-terminal fusion of SsrA tag for recognition by ClpX protease

GFP, human growth hormone, human necrosis factor α, α amylase

[41]

Escherichia coli, Pseudomonas putida

C-Type lysozyme with N-terminal fusion of a signal peptide for secretion

PHA

[40]

Escherichia coli

Toxicity domain of colicin M with N-terminal fusion of PelB signal peptide and SsrA degradation tag

Poly(lactate-co-3-hydroxybutyrate), acetate

[8]

Escherichia coli

Chimeric enzyme ClyN targeting streptococci

Enzyme mutants

[47]

Salmonella enterica serovar Typhimurium

Protein E from E. coli phage X174

Haemolysin E, GFP, cyan fluorescent protein

[11, 14]

Escherichia coli

Protein E from E. coli phage X174

PHA, GFP, β-galactosidase

[9, 55, 56]

Synechocystis sp.

Lipase from Fervidobacterium nodosum Rt17-B1

Membrane lipids

[58]

Synechocystis sp.

Lipase from Staphylococcus hyicus, phospholipase from Fusarium oxysporum, or lipase from guinea pig

Membrane lipids

[59]