Fig. 4

Shake-flask fermentation parameters using mannitol as a carbon source. (A) Cell growth and mannitol-consumption curves of strains with mtlR knockout and mtlTD genes overexpression. (B) L-Tyr production in strains with mtlR gene knocked out and mtlTD genes overexpressed. (C) Cell growth and mannitol-consumption curves of strains by using three different strength promoters to overexpress the pfkB gene. (D) L-Tyr production by using three different strength promoters to overexpress the pfkB gene. (E) Cell growth and mannitol-consumption curves of CCMT04 in different concentrations of mannitol. (F) L-Tyr production of CCMT04 in different concentrations of mannitol