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Table 1 Plasmid copy number (PCN) of subpopulations is significantly different. For normalization we used the EV strain’s single copy gene of the ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit N-methyltransferase I and the Sh ble gene. Statistical comparisons were made using one-way ANOVA and Tukey post-hoc test. Letters denote statistically significant differences between samples with p value < 0.001

From: No two clones are alike: characterization of heterologous subpopulations in a transgenic cell line of the model diatom Phaeodactylum tricornutum

Sample

PCN*

2.04−∆∆Ct

GFP

0.83 ± 0.038a

GFP +

0.89 ± 0.066a

GFP ++

1.80 ± 0.147b

  1. * Average ± S.D. (n = 3)