Table 1 Plasmid copy number (PCN) of subpopulations is significantly different. For normalization we used the EV strain’s single copy gene of the ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit N-methyltransferase I and the Sh ble gene. Statistical comparisons were made using one-way ANOVA and Tukey post-hoc test. Letters denote statistically significant differences between samples with p value < 0.001