Fig. 7

Western blot assay for the identification of PTH84-6xHis peptide forms. Membrane transferred from Tricine SDS-PAGE loaded with samples from BL21(DE3) (pExAB; pET-PTH84) culture. Detection with Anti-His tag antibody. (1): protein ladder (5 µl); (2): 15 µl of supernatant; (3): 15 µl of cell lysate