Fig. 5

Secretion of PTH peptides: supernatants. (A) Tricine SDS-PAGE of supernatants from cultures of control and experimental strains. Aliquots of 10 µl of each supernatant were loaded. (1): 1 µl of PTH84 (1 µg); (2): BL21(DE3) (pExAB; pET-PTH84); (3): BL21(DE3) (pET-PTH84); (4): protein ladder (5 µl); (5): BL21(DE3) (pExAB); (6): BL21(DE3) (pExAB; pET-PTH34); (7): BL21(DE3) (pET-PTH34); (8): 1 µl of PTH34 (1 µg). Asterisks, bands analysed by mass spectrometry. (B) Mass spectrometry analyses from bands marked in A. PTH84-6xHis and PTH34-6xHis sequences were included in the data base of the analysis. Red, peptides detected by mass spectrometry