Fig. 4

Deletion of hupA and/or hupS changes expression of genes associated with production of secondary metabolites (A) Heatmap showing normalized expression (CPM) of the red cluster genes in wild type, ΔhupA, ΔhupS and ΔhupAΔhupS s trains during mid-log and early stationary phase. (B) Production of undecyloprodigiosin (red metabolite) during plate culture of wild type, ΔhupA, ΔhupS and ΔhupAΔhupS s trains incubated of solid SFM medium for 72 h in 30 °C. (C) Heatmap showing normalized expression (CPM) of the carotenoid gene cluster in wild type, ΔhupA, ΔhupS and ΔhupAΔhupS strains during mid-log and early stationary phase. (D) Heatmap showing normalized expression (CPM) of the ectoine gene cluster in wild type, ΔhupA, ΔhupS and ΔhupAΔhupS strains during mid-log and early stationary phase. (E) Heatmap showing normalized expression (CPM) of the desferrioxamine cluster genes in wild type, ΔhupA, ΔhupS and ΔhupAΔhupS strains during mid-log and early stationary phase. A,C,D,E Genes marked with diamond were found to be significantly changed in comparison to the wild type strain (FDR ≤ 0.05). Genes encoding established transcription regulators are marked in grey