Fig. 6
(Run 2) Continuous linalool bioproduction at μ = 0.15 h−1, with an intervening disruption. a OD600 and linalool titres measured for the parental (P) and Δdxr strain (Δ). Compressed air supply was cut off throughout the entire disruption phase, which overlaps with a halt in fresh nutrient influx for the first 16 h. Error bars represent the standard deviation of the technical duplicates, though they may be smaller than the displayed symbols. b At the respective time points (top row), plasmids were normalised to OD600 for extraction, and then digested with NcoI. The DNA ladder is on the leftmost lane. ‘Seed’ refers to the respective seed cultures used for inoculating the reactors. Assignment of the bands to the respective plasmids are indicated on the right. ‘pMVA-Lin (PJ23116::IS10L)’ refers to the IS10-left inserted pMVA-Lin. The two uncut mutant plasmids of the parental strain produced the five-band pattern observed. Refer to Figure S2 for plasmid maps