Fig. 2

Extracellular activities determined by enzymatic hydrolysis of pNPB. Protein expression in E. coli using autoinduction media (Autoinductionsupplemented) in stirred-tank reactors was monitored at 20 °C (blue) and 30 °C (red) expression temperature. Error bars represent the standard deviation (ntechnical = 3), although they may be too small to be visible