Fig. 1

Extracellular activities determined by enzymatic hydrolysis of pNPB using E. coli and C. glutamicum cultures in stirred-tank reactors after 24 h. Different signal peptides were used for the secretion of the target protein in C. glutamicum cultures. For E. coli, induction by autoinduction and IPTG was compared. As controls, C. glutamicum was cultivated with only the vector pEKEx2 and E. coli with the vector pET26b. Each bar represents the mean ± standard deviation of three hydrolysis measurements