Fig. 3

The phylogenetic tree of c21873-1, c23467, twenty-four representative RIPs and MazF protein. By sequence blasting analysis with c21873-1 in NCBI database, c23467 and twenty-four representative RIPs were selected. Then, the amino acid sequences of c21873-1, c23467, twenty-four RIPs and MazF protein were aligned using Clustal W. The phylogenetic tree was constructed by MEGA11 software [41], using the neighbor-joining method with the amino acid substitution model and bootstrapping with 990 iterations. The tree was annotated and displayed using an online service software tvBOT [42]. The name, species and the accession numbers are listed in Table S1