Harnessing the power of Neobacillus niacini AUMC-B524 for silver oxide nanoparticle synthesis: optimization, characterization, and bioactivity exploration

Table 2 Box–Behnken design representing Ag₂ONPs production

Run No	Experimental parameters			Response Ag₂ONPs yield (O.D. at 422 nm)
Run No	A	B	C	Actual values	Predicted values
1	− 1	− 1	0	2.11	1.85
2	+ 1	− 1	0	4.98	4.16
3	− 1	+ 1	0	3.22	4.04
4	+ 1	+ 1	0	3.79	4.05
5	− 1	0	− 1	4.81	5.04
6	+ 1	0	− 1	1.69	2.48
7	− 1	0	+ 1	1.24	0.445
8	+ 1	0	+ 1	5.55	5.32
9	0	− 1	− 1	4.79	4.82
10	0	+ 1	− 1	5.10	4.04
11	0	− 1	+ 1	1.06	2.12
12	0	+ 1	+ 1	5.01	4.98
13	0	0	0	5.28	5.70
14	0	0	0	4.95	5.70
15	0	0	0	6.25	5.70
16	0	0	0	6.02	5.70
17	0	0	0	6.00	5.70

A quadratic polynomial equation that defines predicted response (R) was calculated by the model regression analysis as follows

ISSN: 1475-2859