Fig. 6

Schematic overview of central metabolism and intermediate metabolites of A. woodii [P_bgaL_ldhD_NFP] and C. drakei in co-culture. Both strains carry genes encoding for the Wood-Ljungdahl-Pathway (WLP) for carbon fixation. The simplified WLP reactions are balanced for 1 mol acetyl-CoA while the chemiosmotic energy balancing and respiratory systems are only balanced for the respective enzyme, not the total WLP. Hyt forms a complex with the formate dehydrogenase of C. drakei (see text). Enzyme complex abbreviations: Rnf, membrane bound Fd-NAD⁺ oxidoreductase complex; ATPase, ATP synthase; HydABC, soluble [FeFe]-hydrogenase; Hyt, NADP⁺-specific electron bifurcating [FeFe]-hydrogenase; Nfn, electron-bifurcating transhydrogenase complex; CoFeSP, corrinoid iron sulfur protein; Fd²⁻, reduced ferredoxin. Grey arrows denote extracellular transport and metabolite exchange between the two strains