Table 1 Plasmids used in this study
From: Metabolic engineering of Komagataella phaffii for the efficient utilization of methanol
Plasmids | Genotype | Source or reference |
|---|---|---|
PPIC9K | KanR | Invitrogen |
PPICZA | ZeocinR | Invitrogen |
PPICKSTD | KanR, PAOX1-Spytag-Das1-TAOX1 | This study |
PPICZCSCA | ZeocinR; PAOX1-Cre-TAOX1; PAOX1-Spycather-Aox1-TAOX1 | This study |
PHKAM | KanR, PAOX1-MDH-TAOX1 | This study |
PHKAI | KanR, PAOX1-IDH-TAOX1 | This study |
PHKAP | KanR, PAOX1-FBP-TAOX1 | This study |
PHKAA | KanR, PAOX1- FBA-TAOX1 | This study |
PHKAD | KanR, PAOX1- DAK-TAOX1 | This study |
PHKAT | KanR, PAOX1-TAL-TAOX1 | This study |
PHKAR | KanR, PAOX1-RPIA-TAOX1 | This study |
PPICZAG | ZeocinR, PAOX1-based expression vector; | This study |
PPICZAGMP | ZeocinR, PAOX1-MDH-TAOX1; PAOX1-FBP-TAOX1 | This study |
PPICZAGIP | ZeocinR, PAOX1-IDH-TAOX1; PAOX1-FBP-TAOX1; | This study |
PPICZAGMIP | ZeocinR, PAOX1-MDH-TAOX1; PAOX1-IDH-TAOX1; PAOX1-FBP-TAOX1 | This study |
PPICZAGIPMOX | ZeocinR, PAOX1- IDH-TAOX1; PAOX1-FBP-TAOX1; PAOX1-MOX -TAOX1 | This study |