Fig. 5
From: Metabolic engineering of Komagataella phaffii for the efficient utilization of methanol
Analysis of strains with enzyme overexpression with NAD+ as cofactor was incubated in 25 mL of BMMY medium containing 1% methanol. A Schematic diagram of TCA cycle and malate shuttle system, Mdh, malate dehydrogenase; Idh, isocitrate dehydrogenase; MAL, malate; OXA, oxaloacetate; ISO, isocitrate; OXAL, oxalosuccinate; B Schematic diagram of expression plasmid; C Measurement of growth curve; The x-axes of the culture plots start at 18 h. D Measurement of intracellular NAD+/NADH; E Measurement of methanol in fermentation supernatant. Error bars represent the standard deviation of 2 or 3 biological replicates