Fig. 4

Analysis of strains with overexpression of key enzymes of the XuMP pathway were incubated in 25 mL of BMMY medium containing 1% methanol. A Schematic diagram of the methanol assimilation metabolic pathway, Aox, alcohol oxidase; Das, dihydroxyacetone synthase; Dak, dihydroxyacetone kinase; Fba fructose-bisphosphate aldolase; Fbp, fructose bisphosphatase; Tpi, triosephosphate isomerase; Tal, transaldolase; Rpi, ribose-5-phosphate isomerase; Rpe ribulose phosphate 3-epimerase; GAP, glyceraldehyde 3-phosphate; DHA, dihydroxyacetone; DHAP, dihydroxyacetone phosphate; F1,6BP, fructose-1 6-bisphposphate; F6P, fructose 6 phosphate; E4P, erythrose-4-phosphate; SBP, sedoheptulose 1,7-bisphosphate; S7P, sedoheptulose 7-phosphate; Xu5P, xylulose 5-phosphate; R5P, ribose 5-phosphate; Ru5P, ribulose 5-phosphate; B Schematic diagram of the overexpression plasmid; C Measurement of 48 h growth OD₆₀₀; D Measurement of 48 h fermentation supernatant methanol; E Measurement of fermentation supernatant formaldehyde. Error bars represent the standard deviation of 2 or 3 biological replicates