Fig. 6
A Growth of E. coli W ΔcscR and E. coli BL21 (DE3) in the presence 10 mM sucrose in M9 minimal medium. Reaction conditions: 30 mL volume in 100 mL baffled flasks, 37 °C, 120 rpm; and B Specific enzyme activities obtained in whole-cell biotransformation of 1a mediated by BVMOXeno::cscA and BVMOXeno::pelB_cscA produced in E. coli W ΔcscR and E. coli BL21 (DE3) when supplemented with 10 mM sucrose. Reaction conditions: 30 mL, M9 salts as medium, 1.5 gDCW L−1, 5 mM 1a, 25 °C, 160 rpm, N = 3. All values represent data generated from three biological replicates. Error bars represent standard deviations. P-values were calculated using Welch’s t test (*P < 0.05)