Fig. 4

Whole-cell biotransformation of 1a mediated by different E. coli BL21 (DE3) constructs harboring BVMOXeno in the presence of sugars (2 mM glucose or 1 mM sucrose). Reaction conditions: 30 mL, M9 salts as medium, 1.5 gDCW L−1, 5 mM 1a, 25 °C, 160 rpm, N ≥ 3. All bars represent data generated from reactions stemming from biological replicates with individual values depicted. Error bars represent standard deviations. P values were calculated using Welch’s t test (*P < 0.05)