CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome

Table 1 Engineered strains with reduced secreted proteins

Gene	Function	S∆3a	S∆3b	S∆6	S∆11
TOS1	Cell wall protein	∆		∆	∆
CRH1	Chitin transglycosylase	∆		∆	∆
EXG1	Beta-glucanase	∆		∆	∆
GQ67_05326	Unknown		∆	∆	∆
LSC2	Succinyl CoA ligase		∆	∆	∆
TFS1	Vacuole targeting		∆	∆
GQ67_02848	Unknown				∆
TEF4	Translation factor				∆
CTS1	Chitinase				∆
GQ67_01368	Unknown				∆
GQ67_03274	Unknown				∆
MSC1	Unknown				∆

ISSN: 1475-2859