Fig. 3

Productivity and growth of engineered knockout strains. A Secreted titer and specific productivity of HSA from 3 mL microplate cultures. B SDS-PAGE of 3 mL microplate culture supernatant. HSA protein is visible at ~70 kDa. C Enriched gene sets between S∆6 and the base strain during production of albumin. D Secreted titer and specific productivity of trastuzumab in 3 mL microplate cultures. E Secreted titer and specific productivity of trastuzumab in 100 mL shake flask cultures. F Enriched gene sets between S∆6 and the base strain during production of trastuzumab. G Growth rates of strains in 200 µL cultures in glycerol outgrowth medium. H Growth rates of strains in 200 µL cultures in methanol production medium. In all bar plots, significance of specific productivity or exponential growth rate compared to the base strain was determined by unpaired Welch’s t-test