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Table 1 Proteins produced in this work

From: Producing recombinant proteins in Vibrio natriegens

Expressed protein

Purified protein

Yield (mg/L)*

E. coli

V. natriegens

FLAG-Mm.AMHR2(18–142)-His6

FLAG-Mm.AMHR2(18–142)-His6

nd1

7.0 +/- 3.3

Nanobody RBD-1-1G

RBD-1-1G

nd2

7.5

Nanobody RBD-1-1E

RBD-1-1E

nd2

36.6

Nanobody RBD-1–3 H

RBD-1–3 H

nd2

5.3

Nanobody RBD-1-2G

RBD-1-2G

0.1, 0.004

2.5 +/- 0.4

His6-MBP-tev-GG-Hs.KRAS4b(2-169)

GG-Hs.KRAS4b(2-169)

29 +/- 7

41 +/- 4

His6-MBP-tev-G-Hs.NRAS(1-169)

G-Hs.NRAS(1-169)

6 +/- 2

25 +/- 14

His6-MBP-tev-RAF1(52–192)

Hs.RAF1(52–192)

7 +/- 1

51 +/- 22

His6-MBP-tev-RAF1(52–192) 15N

Hs.RAF1(52–192) 15N

0.6 +/- 0.5

1.4 +/- 0.3

His6-MBP-tev-G-Hs.KRAS4b(1-169) D2O

G-Hs.KRAS4b(1-169) D2O

2.7, 1.93

3.8 +/- 0.6

  1. * When a standard deviation is reported, a minimum of three independent productions were performed
  2. 1 Due to the lack of expressed protein, no scale up was possible
  3. 2 Due to the poor prognosis at small-scale screen, no scale up was attempted in E. coli. A single production was produced in V. natriegens
  4. 3 Result from two independent productions
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Microbial Cell Factories

ISSN: 1475-2859

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