Table 1 Strains and plasmids used in this study

Strains

E. coli DH5α

the cloning host

This lab

E. coli MG1655 ΔlacI

the starting strain

This lab

MQ1

E. coli MG1655, ΔlacI, yghX::P_trc-BspurEK-T_{BBa_B1006}

This study

MQ2

MQ1, ypjC-ileY::P_trc-BspurBCSQ-T_{BBa_B1002}

This study

MQ3

MQ2, fliK::P_trc-BspurLF-T_{BBa_B1004}

This study

MQ4

MQ3, lacZ::P_trc-BspurMNHD-T_{rrnB T2}

This study

MQ5

MQ4, aslA-glmZ::P_trc-prs -T_{BBa_B1005}

This study

MQ6

MQ4, aslA-glmZ::P_trc-Ecprs^D128A-T_{BBa_B1005}

This study

MQ7

MQ4, aslA-glmZ::P_trc-Baprs-T_{BBa_B1005}

This study

MQ9

MQ7, ΔdeoD

This study

MQ14

MQ9, ΔppnP

This study

MQ15

MQ9, Δgsk

This study

MQ16

MQ9, ΔrihA

This study

MQ17

MQ9, ΔrihB

This study

MQ18

MQ9, ΔrihC

This study

MQ19

MQ14, Δgsk

This study

MQ20

MQ19, ΔpurA

This study

MQ21

MQ19, ΔpurA::P_trc-BspurA^P242N-T_{BBa_B1003}

This study

MQ23

MQ19, purA:: purA-ssrA

This study

MQ24

MQ23, ΔpurR

This study

MQ25

MQ24, ΔpfkA

This study

MQ26

MQ25, ΔpfkA::P_J23116-glpX-T_{BBa_B1003}

This study

MQ27

MQ25, ΔpfkA::P_J23116-BsglpX-T_{BBa_B1003}

This study

MQ28

MQ27, Δeda, Δedd

This study

MQ29

MQ28, ΔpntAB

This study

MQ30

MQ28, ΔyghE::P_trc-sthA-T_{BBa_B1010}

This study

MQ31

MQ28, ΔpntAB::P_trc-sthA-T_{BBa_B1010}

This study

MQ32

MQ28, ΔnupG

This study

MQ33

MQ28, ΔnupG::P_J23108-nepI-T_{BBa_B1003}

This study

MQ34

MQ28, ΔnupG::P_J23108-BspbuE-T_{BBa_B1003}

This study

MQ38

MQ33, ykgH-betA::P_tac-guaAB

This study

MQ39

MQ33, ΔguaC::P_tac-guaAB

This study

Plasmids

#1

Kan^R, Cas9 and λ-Red recombinase expression vector

[21]

#2

Amp^R, gRNA expression vector

[21]