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Table 1 Strains used in this study

From: Citrate synthase variants improve yield of acetyl-CoA derived 3-hydroxybutyrate in Escherichia coli

Strain

Relevant characteristics

Reference

ATCC 9637

Escherichia coli W

Wild-type

MEC1316

ATCC 9637 ΔpoxB ΔldhA

[79]

MEC1353

ATCC 9637 ΔgltA

This study

MEC1365

MEC1316 ΔpoxB ΔldhA Δpta-ackA

This study

MEC1381

MEC1365 ΔgltA

This study

MEC1394

MEC1381 ΔgltA::gltA[A267T]

This study

MEC1410

MEC1381 ΔgltA::gltA[F383M]

This study

MEC1411

MEC1381 ΔgltA::gltA[K167A]

This study

MEC1482

MEC1381 ΔgltA::gltA[V361A]

This study

MEC1567

MEC1381 ΔgltA::gltA[M372S]

This study