Table 3 Minimum bacteriostatic concentration (MIC), Minimum bactericidal concentration (MBC), MBC/MIC ratio and the antimicrobial activity of pyocyanin extract against tested Gram-positive, Gram-negative bacteria
Gram Type | Bacterial strain | MIC (µg/mL) | MBC (µg/mL) | MBC/MIC ratio | Antibacterial Effect (Bactericidal/Bacteriostatic) |
|---|---|---|---|---|---|
Gram Positive | S. aureus ATCC 25,923 | 62.5 | 125 | 2 | Bactericidal |
MSSA | 62.5 | 250 | 4 | Bactericidal | |
MRSA (Swab from abdominal wall) | 62.5 | 500 | 8 | Bacteriostatic | |
MRSA (sputum) | 125 | 1000 | 8 | Bacteriostatic | |
MRSA (Swab from gluteal abscess) | 125 | 1000 | 8 | Bacteriostatic | |
S. epidermidis (urine) | 62.5 | > 4000 | > 4 | Bacteriostatic | |
S. epidermidis (aspirate) | 62.5 | > 4000 | > 4 | Bacteriostatic | |
S. saprophyticus | 125 | 250 | 2 | Bactericidal | |
S. haemolyticus | 125 | 2000 | 16 | Bacteriostatic | |
S. agalactiae | 125 | 4000 | 32 | Bacteriostatic | |
S. pyogenes | 125 | 4000 | 32 | Bacteriostatic | |
Bacillus sp.1 | 31.25 | 125 | 4 | Bactericidal | |
Bacillus sp.2 | 125 | 500 | 4 | Bactericidal | |
Bacillus sp.3 | 62.5 | 62.5 | 1 | Bactericidal | |
L. monocytogenes ATCC 35,152 | 2000 | > 4000 | Not detected | Not detected | |
E. faecalis ATCC 29,212 | 2000 | > 4000 | Not detected | Not detected | |
Gram Negative | E. coli ATCC 25,922 | 250 | > 4000 | > 4 | Bacteriostatic |
E. coli ATCC 8753 | 500 | > 4000 | > 4 | Bacteriostatic | |
ESBL E. coli (Aspirate) | 500 | > 4000 | > 4 | Bacteriostatic | |
ESBL E. coli (Urine) | 1000 | > 4000 | > 4 | Bacteriostatic | |
K. pneumoniae ATCC 10,031 | 4000 | > 4000 | Not detected | Not detected | |
ESBL K. pneumonia | 4000 | > 4000 | Not detected | Not detected | |
A. baumannii | 4000 | > 4000 | Not detected | Not detected | |
P. mirabilis | 4000 | > 4000 | Not detected | Not detected | |
S. typhimurium ATCC 14,028 | 2000 | > 4000 | Not detected | Not detected | |
P. aeruginosa ATCC 27,853 | No inhibition detected | Not tested | Not detected | No activity detected |